The kinetics of DEAE-dextran-induced cell sensitization to transfection.
نویسندگان
چکیده
منابع مشابه
DEAE-dextran enhances electroporation of mammalian cells.
Electroporation has proven to be a convenient method for transfection of many cell types. One drawback has been that it requires large amounts of DNA, typically 20 to 80 /tg per transfection (1). Here we report that adding DEAE-dextran to the electroporation media dramatically increases electroporation efficiency in mammalian cells, lowering the amount of DNA required for efficient transfection...
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As a novel target for breast cancer, interferon inducers have found its role as anti-angiogenic agents with diethylaminoethyl dextran (DEAE-Dextran) being a molecule used for centuries as a transfection agent. Our results herein offer an explanation for the emergence of DEAE-Dextran as an anti-tumor agent for TNBC with in-depth mechanistic approach as an anti-angiogenic molecule. DEAE-Dextran h...
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Using a plasmid containing the bacterial chloramphenicol acetyl transferase gene, we have assayed for transient expression of DNA introduced into mouse L cells by a variety of transfection conditions. High efficiency uptake and expression of this foreign DNA have been achieved by modifying the DEAE dextran mediated transfection procedure of McCutchan and Pagano (1) to include a shock with eithe...
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ورودعنوان ژورنال:
- The Journal of general virology
دوره 18 2 شماره
صفحات -
تاریخ انتشار 1973